Adel T. M. Al- Saeed
College of Medicine, University of Duhok, Kurdistan, Iraq
Title: Molecular detection of Cryptosporidiosis in Children in Duhok City / Kurdistan Region/Iraq
Biography:
Al-Saeed is professor in Molecular Parasitology. He has his expertise in teaching the medical parasitology for undergraduate and postgraduate students more than 30 years. He was supervised on 9 M.Sc. and 4 Ph.D. students.
Abstract:
The study was performed to detect Cryptosporidium in children that attended Hevi Teaching Pediatric Hospital in Duhok city/Kurdistan region of Iraq. Stool samples were collected from children with or without diarrhea included both sexes and different age groups ranging from less than 1 – 12 year old.
A total of 122 stool samples collected from children were examined to detect Cryptosporidium by conventional PCR that carried out at Duhok Research Center in the college of Veterinary Medicine.
Out of 122 stool samples of children of which 48 (39.34%) were positive against cryptosporidiosis. The samples were categorized according to diarrheic and non-diarrheic
In diarrheic children, the highest infection rate 7/9 (77.78%) was among those who 9-12 year old, whereas the lowest infection rate 5/19 (26.32%) was among the age group 5-8 year. In non-diarrheic children the highest infection rate 4/11 (36.36%) was among 1-4 year, while the lowest infection rate 3/15 (20%), 2/10 (20%) were among the both age group of 5-8 year and 9-12 year respectively.
This study indicates that asymptomatic infection is common and could be detected by PCR among different age groups of children. In the present study, the results of sequences analyzing of PCR products; showed identical to the Cryptosporidium sequences in the GenBank.