Speaker Biography

Dr HOWAIDA ISMAIL

Dr HOWAIDA ISMAIL,,Medical ParasitologyDepartment,Faculty of Medicine,Tanta University ,Egypt.

Title: IMPACT OF IMMUNE SUPPRESSION ON HISTOPATHOLOGICAL AND IMMUNOLOGICAL PARAMETERS OF MICE EXPERIMENTALLY INFECTED WITH GIARDIA LAMBLIA

Biography:

 Professor Howaida I.H. Ismail is a professor of Medical Parasitology at Faculty of Medicine,Tanta University,Egypt. She had Master Degree in Medical Parasitology(1992) and M.D.Degree in Medical Parasitology(1997)  . In addition ,she had Master Degree in Microbiology and Immunology(2009) – Faculty of Medicine,Tanta University . She is head of Parasitology Department,Faculty of Medicine,Tanta University. Her research interests include Medical Parasitology (Helminths,Protozoa).

 

Abstract:

Giardiasis is one of the most frequent entero-parasites worldwide; its prevalence is more

 common in developing countries. Giardia lamblia is considered one of the opportunistic para-sites and its clinical manifestations represent the expression of host resistance and parasite viru-lence. As of chronic giardiasis cases were expected to worsen in immunosuppressed patients with development of various complications. This work assessed the histolopathological and the possible immunological effects of infection with G. lamblia in an immunosuppressed experi-mental animal model in comparison to immunocompetent one. Mice were divided into 4 groups: group I: immunocompetent (IC) mice infected with G. lamblia cysts, group II: immunosup-pressed (IS) mice infected with cysts, group III: uninfected immunocompetent mice and group IV: uninfected immunosuppressed mice. From each group, small intestine was removed for his-topathological and molecular studies. Also, cyst counting in the stool of infected mice was esti-mated. We found that the number of G. lamblia cysts in the stool of IS mice was significantly higher than that from IC ones. Shortening of the villi was more pronounced in the IS than in IC group. Furthermore, intraepithelial lymphocytic count, goblet cell count and mast cell count were significantly decreased in the IS infected group as compared to IC ones. Expression of in-terleukin-6 mRNA showed high expression in IC infected mice while it was weak in IS mice. In conclusion, the present study revealed the importance of the innate immunity presented by goblet cells, mast cells and other adaptive immunity responses; in the clearance of Giardia.