Ram Bahadur Khadka
Pokhara University, Nepal
Title: Screening of NS1 antigen and early diagnosis of dengue in patients visiting Crimson Hospital, Nepal
Biography:
Ram Bahadur Khadka is a Senior Microbiologist at Crimson College of Technology where he worked as a lecturer of Health Science (Bsc.MLT) including laboratory Technologist to hold all critical conditions at medical laboratory fields. He has worked with different laboratories and medical college as a leading person. He helps to solve and handle upcoming laboratory professional problems to provide quality and effective reports for patients. He is a microbiologist form Butwal – 12, Province no – 5, Nepal.
Abstract:
Introduction:
Dengue an arboviral disease is one of the foremost important arthropod borne manifestation which is highly prevalent in tropical and subtropical regions of the world. It is estimated that annually around fifty million populations are infected with dengue globally.Clinical manifestations of dengue includes from mild fever to severe life threatening complications like dengue hemorrhagic fever and dengue shock syndrome.
Objective:
The objective of this study was to determine the prevalence of dengue disease among the suspected individuals in a tertiary care setting.
Materials and Methods:
A retrospective study was done from March 2019 to May 2019 among 821 participations in Crimson Hospital, Butwal, Nepal. Blood samples were collected and World Health Organization Good Manufacturing Practices (GMP) certified rapid solid phase immune-chromatographic method was used for qualitative detection of Dengue Non – Structural Protein 1antigen (NS1), Anti IgM and Anti IgG antibody following standard protocol. Collected data were tabulated in MS-Excel and analyzed by using Statistical Package for the Social Sciences software version 20.
Result: Out of 821 study participants 389 (47.4%) were Positive for NS1test and was more prevalent among the age group of 21 to 30 years, whereas 175 (21.3%) and 11(1.3%) were positive for IgM and IgG antibodies respectively.
Conclusions: Our study revealed the high prevalence of dengue cases, so the methods for early diagnosis, steps for awareness of prevention, better case management and faster public health response is must to reduce the disease burden.