International Conference on
Clinical Microbiology and Immunology

Biography:

Abstract:

STATEMENT OF PROBLEM: Malaria is one of the killer diseases in Nigeria, according to WHO and UNICEF; the disease claims a higher percentage of infants’ populations in the countries every year. Government spend huge amount on drugs for large amount of money could be reduced if this saraca tea can be taking regularly which shows no negative side effective on human system unlike the synthetic drugs produced

METHODOLOGY

This was done in two ways                    

 (i) Chemical extraction using a universal solvent

(ii) Local extraction using not water

The   leaves were collected from the tree and sun-dried for 3 day so as reduces the moisture content of the after drying is then grinded into powdery form so as to give large surface area during extraction. After grinding, the power is then put into a soxhlet extraction chamber and using a universal solvent and allow to stay for about 3 days or heated to extract the active juice  which settles in the bottom of the soxhlet extractor

The extract was then filtered so as to remove any residue from the extract and the solvent is then stored in a jar as solution.

The powdered leaf was also packaged in a tea-bag  and was drawn in a hot cup of water before testing on animals, the phytochemical analysis was perform in the laboratory. The extract was tested by inoculating a white mouse with blood sample known to contain plasmodium falciparium and allow to incubate for at least 3 day, the blood sample of the test mouse was then drawn and view under microscope for the presence of plasmodium falciparium and later injected with the extract of both method. The extract introduce according to the weight of the mouse. For 3 day twice a day. Also the saraca indic ita tea was tested by soaking a bag of the saraca tea in a teacup containing hot water to be taking twice daily at 8 hours interval by diseased individual at selected in 3 states Lagos, Ogun and Oyo. The blood sample of mouse and individual was then taken and examined again under the microscope using this method

Examination of blood sample

Blood smear on slide+ Giemsa stain => parasitized red blood cells gives a pink coloration.

RESULT

After the mouse has been injected with blood sample containplasmodium falciparium the table below shows the observable parasitized red blood cell (RBC) which shows pink colorations upon viewing.

It was observed that the extract is very effective against malaria parasite which has been discussed on one of the most deadly disease in Africa according to WHO and UNICEF which has consumed much of life and goverment money for the past years. It is assumed that if this research is allow to see the light of the day, it will reduce a lot govt. spending and rate of mortality of infants and adults