International Conference on
Clinical Microbiology and Immunology

Biography:

Abstract:

Escherichia coli (E. coli) are the most common cause of both hospital and community acquired urinary tract infection. Studies on molecular characterization of extended spectrum beta-lactamase (ESBL) resistant E. coli from urine of patients attending tertiary Health Care Centre, Nasarawa State, Nigeria was carried out. A total of 780 (380 from Federal Medical Centre Keffi [FMCK] and 400 from Dalhatu Araf Specialist Hospital Lafia [DASHL]) urine samples from patients with suspected urinary tract infections were collected over a period of six (6) month; and E. coli was isolated and identified using standard microbiological methods. Antimicrobial susceptibility testing for the isolates was carried out in accordance with Clinical and Laboratory Standards Institute protocol. The detection of ESBL production in isolates resistant to third generation cephalosporins (cefotaxime and ceftazidime) was carried out using double disc synergy test. The detection of ESBL resistance genes in phenotypic ESBL producing isolates was carried out using Polymerase chain reaction method. The overall occurrence of the isolates was 107 (13.7%). The occurrence of the isolates in relation to the hospital was high in DASHL (13.7%) than FMCK (12.7%). The occurrence of isolates in relation to gender of patients was high in female both at FMCK (15.5%) and DASHL (15.8%). The occurrence of the isolates in relation to age was both high in 11-20 years (23.5%) at FMCK and ˃50 years (31.8%) at DASHL. The isolates in DASHL were more resistant to ampicillin (81.6%) and streptomycin and sulphamethoxazole/trimethoprime (75.0%) while in FMCK, the isolates were also more resistant to ampicillin (80.4%) and streptomycin (68.2%). The overall occurrence of ESBL producing isolates was 18 (54.5%). The occurrence of the ESBL producing isolates in relation to the hospitals was high in DASHL (54.5%) than FMCK (46.2%). The occurrence of ESBL genes such as bla_TEM was high in DASHL (66.67%) while bla_SHV and bla_CTX-M were high in FMCK (83.33%) but bla_CTX-M was low in DASHL (33.3%). Most of the isolates both in the tertiary hospitals were more resistance to the antibiotics commonly but gentamicin, imipenem and amoxicillin/clavulanic were effective against the isolates. Most of the isolates were also ESBL resistance and the predominant ESBL resistance genes were bla_TEM and bla_CTX-M in both hospitalsThe antibiotics such as gentamicin, imipenem and amoxicillin/clavulanic acid should be used for treatment UTIs caused by E. coli isolates.